1PYS
PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE |
| Synchrotron site | LURE |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 176.000, 176.000, 141.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.23600 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 25.441 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 52260 | |
| <I/σ(I)> | 13.1 | 3 |
| Completeness [%] | 92.9 | 75.2 |
| Redundancy | 4.6 | 2.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.7 | 4 * | Chernaya, M., (1987) J. Mol. Biol., 198, 555. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-5 (mg/ml) | |
| 2 | 1 | drop | imidazole-HCl | 20 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | ammonium sulfate | 15 (%sat) | |
| 6 | 1 | reservoir | ammonium sulfate | 25-30 (%sat) | |
| 7 | 1 | reservoir | imidazole-HCl | 20 (mM) |
