1PY6
Bacteriorhodopsin crystallized from bicells
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-08-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.383, 108.532, 55.796 |
| Unit cell angles | 90.00, 113.43, 90.00 |
Refinement procedure
| Resolution | 19.160 - 1.800 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 18.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.160 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.103 | 0.295 |
| Number of reflections | 41395 | |
| <I/σ(I)> | 12.6 | 1.97 |
| Completeness [%] | 92.4 | 59.5 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 3.7 * | 310 | DMPC, Chapso, Sodium Phosphate, 1,6 Hexane Diol, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | DMPC/CHAPSO | 40 (%) | 2.8:1 |
| 3 | 1 | reservoir | sodium potassium phosphate | 2.4 (M) | pH3.7 |
| 4 | 1 | reservoir | 1,6-hexanediol | 120 (mM) |
