1PY4
Beta2 microglobulin mutant H31Y displays hints for amyloid formations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Wavelength(s) | 0.927 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 105.195, 150.166, 93.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.900 |
R-factor | 0.24084 |
Rwork | 0.240 |
R-free | 0.31308 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lds |
RMSD bond length | 0.014 |
RMSD bond angle | 1.631 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 16354 |
<I/σ(I)> | 19.8 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | PEG 4K 31%, glycerol 25%, ammonium acetate 0.2 M, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | PEG 4K 31%, glycerol 25%, ammonium acetate 0.2 M, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |