1PY4

Beta2 microglobulin mutant H31Y displays hints for amyloid formations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Wavelength(s)	0.927
Spacegroup name	C 2 2 21
Unit cell lengths	105.195, 150.166, 93.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.900
R-factor	0.24084
Rwork	0.240
R-free	0.31308
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lds
RMSD bond length	0.014
RMSD bond angle	1.631
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.900
Number of reflections	16354
<I/σ(I)>	19.8
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	300	PEG 4K 31%, glycerol 25%, ammonium acetate 0.2 M, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K
1	VAPOR DIFFUSION, HANGING DROP	5.6	300	PEG 4K 31%, glycerol 25%, ammonium acetate 0.2 M, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K