1PY3
Crystal structure of Ribonuclease Sa2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-04-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9185 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.290, 68.700, 57.510 |
| Unit cell angles | 90.00, 100.40, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.17484 |
| Rwork | 0.172 |
| R-free | 0.21825 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgg |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.832 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.052 | 0.378 |
| Number of reflections | 35955 | |
| <I/σ(I)> | 20.5 | 2.96 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 3.45 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | ammonium sulfate, phosphate buffer, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
