1PXA

CRYSTAL STRUCTURES OF MUTANT PSEUDOMONAS AERUGINOSA P-HYDROXYBENZOATE HYDROXYLASE: THE TYR201PHE, TYR385PHE, AND ASN300ASP VARIANTS

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	71.880, 146.620, 88.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.300
R-factor	0.193
Rwork	0.193
RMSD bond length	0.019
RMSD bond angle	3.440
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.121 *
Number of reflections	20384 *
Completeness [%]	97.0 *
Redundancy	4.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate
2	1	1	phosphate	0.1 (M)