1PX3

E. COLI (LACZ) BETA-GALACTOSIDASE (G794A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-11-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	149.700, 168.590, 200.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.700 - 1.600
R-factor	0.192
Rwork	0.192
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dp0
RMSD bond length	0.017
RMSD bond angle	2.800
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.700	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.048	0.300
Number of reflections	610095
<I/σ(I)>	22.6	3.3
Completeness [%]	92.1	81.8
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	PEG 8000, NACL, MGCL2, DTT, BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K