1PX2
Crystal Structure of Rat Synapsin I C Domain Complexed to Ca.ATP (Form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2000-07-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.942 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 96.000, 96.000, 305.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.230 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aux Ca and ATP-gamma-S removed |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.310 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.082 * | 0.391 * |
| Total number of observations | 467389 * | |
| Number of reflections | 40542 * | |
| <I/σ(I)> | 29.9 | 6.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.2 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 299 | PEGMME 5K, Tris, NaCl, Ca.ATP, EDTA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 299K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | drop | calcium ATP | 10 (mM) | |
| 3 | 1 | reservoir | Tris | 150 (mM) | pH7.5 |
| 4 | 1 | reservoir | PEG5000 MME | 15-20 (%(w/v)) | |
| 5 | 1 | reservoir | 300 (mM) |
