1PWE
Rat Liver L-Serine Dehydratase Apo Enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-19 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.628, 169.843, 95.998 |
| Unit cell angles | 90.00, 92.75, 90.00 |
Refinement procedure
| Resolution | 23.600 * - 2.800 |
| Rwork | 0.218 |
| R-free | 0.25000 * |
| Structure solution method | SIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 * | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 * | 0.139 * |
| Total number of observations | 617721 * | |
| Number of reflections | 22269 * | |
| <I/σ(I)> | 2655.8 | 727.4 |
| Completeness [%] | 89.1 | 77.5 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 26 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.0 |
| 2 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 3 | 1 | reservoir | PEG3350 | 2 (%(w/v)) | |
| 4 | 1 | reservoir | ethylene glycol | 20 (%(v/v)) | |
| 5 | 1 | drop | protein | 10 (mg/ml) |
