1PWE

Rat Liver L-Serine Dehydratase Apo Enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-08-19
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	70.628, 169.843, 95.998
Unit cell angles	90.00, 92.75, 90.00

Refinement procedure

Resolution	23.600 * - 2.800
Rwork	0.218
R-free	0.25000 *
Structure solution method	SIR
RMSD bond length	0.009
RMSD bond angle	22.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000 *	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.070 *	0.139 *
Total number of observations	617721 *
Number of reflections	22269 *
<I/σ(I)>	2655.8	727.4
Completeness [%]	89.1	77.5
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	26 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	50 (mM)	pH7.0
2	1	reservoir	dithiothreitol	1 (mM)
3	1	reservoir	PEG3350	2 (%(w/v))
4	1	reservoir	ethylene glycol	20 (%(v/v))
5	1	drop	protein	10 (mg/ml)