1PWB

High resolution crystal structure of an active recombinant fragment of human lung surfactant protein D with maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-03
Detector	ADSC QUANTUM 4
Wavelength(s)	1.244
Spacegroup name	P 1 21 1
Unit cell lengths	55.480, 108.300, 55.780
Unit cell angles	90.00, 91.43, 90.00

Refinement procedure

Resolution	32.100 - 1.400
R-factor	0.205
Rwork	0.205
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b08
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.043 *	0.245 *
Total number of observations	648490 *
Number of reflections	122387	14247 *
<I/σ(I)>	9.2	3
Completeness [%]	94.9	76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	298	PEG 10000, Tris, maltose, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	Tris	10 (mM)
3	1	drop		140 (mM)
4	1	drop		10 (mM)	pH7.5
5	1	reservoir	PEG10000	12 (%)
6	1	reservoir	Tris	10 (mM)
7	1	reservoir	maltose	0.1 (M)	pH6.0