1PW5

putative nagD protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	150
Detector technology	CCD
Collection date	2002-07-26
Detector	SBC-2
Wavelength(s)	0.97929,0.97918,0.96396
Spacegroup name	P 41 21 2
Unit cell lengths	97.788, 97.788, 159.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.930 - 2.800
Rwork	0.244
R-free	0.27900
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	d*TREK
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.930
High resolution limit [Å]	2.800
Number of reflections	18344

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	amonium sulfate, sodium cacodylate, ethylene glycol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K