1PW3
Crystal structure of JtoR68S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 72.247, 72.247, 94.256 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.21981 |
| Rwork | 0.219 |
| R-free | 0.23339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cdo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.190 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 17408 | |
| Completeness [%] | 95.0 | 95.1 |
| Redundancy | 3.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.1M Sodium Acetate, 0.8M Ammonium Sulfate, 40mM Cadmium Chloride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
