1PVT
Crystal structure of sugar-phosphate aldolase from Thermotoga maritima
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-25 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 108.510, 108.510, 53.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.340 - 2.500 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.29600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.063 | 0.630 |
| Number of reflections | 22513 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 93.2 | 39.3 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | ammonium sulfate, sodium chloride, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
