1PVI

STRUCTURE OF PVUII ENDONUCLEASE WITH COGNATE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 21
Unit cell lengths	95.800, 86.300, 48.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.189
Rwork	0.189
RMSD bond length	0.012
RMSD bond angle	1.700 *
Data reduction software	MADNES
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.600 *
Rmerge	0.070
Total number of observations	45607 *
Number of reflections	10282 *
Completeness [%]	92.7 *
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	16 *	Balendiran, K., (1994) Proteins: Struct.,Funct., Genet., 19, 77. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	R-PvuII	9.6 (mg/ml)
2	1	drop	duplex DNA	1.6 (mg/ml)
3	1	drop	mother liquor	18-25 (%)
4	1	drop	PEG4000	8 (%)	mother liquor
5	1	drop	EDTA	0.3 (mM)	mother liquor
6	1	drop	sodium acetate	50 (mM)
7	1	reservoir	mother liquor	31-45 (%)