1PVG

Crystal Structure of the ATPase region of Saccharomyces Cerevisiae topoisomerase II

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-16
Detector	ADSC QUANTUM 4
Wavelength(s)	1.127, 0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	58.456, 71.081, 216.132
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.800
R-factor	0.20866
Rwork	0.206
R-free	0.24100 *
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	0.996 *
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.061	0.430
Total number of observations	1143715 *
Number of reflections	75706
<I/σ(I)>	22	2.8
Completeness [%]	89.5	72.7
Redundancy	6.7	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *	4 *	PEG 1500, Potassium Chloride, Glycerol, Sodium Cacodylate, pH 6.5, microbatch under oil, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	12 (mg/ml)
2	1	2	Tris-HCl	5 (mM)	pH7.5
3	1	2		100 (mM)