1PV9
Prolidase from Pyrococcus furiosus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-03-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.282, 1.283, 1.240, 1.333 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.476, 97.227, 69.882 |
| Unit cell angles | 90.00, 97.09, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.24637 |
| Rwork | 0.245 |
| R-free | 0.28000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 0.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.035 | 0.334 |
| Number of reflections | 51843 | |
| <I/σ(I)> | 26.8 | 2.98 |
| Completeness [%] | 94.9 | 94.1 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 8000, magnesium acetate, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 6.5-7.5 (%) | |
| 3 | 1 | drop | magnesium acetate | 0.1 (M) | |
| 4 | 1 | drop | Tris-HCl | 50 (mM) | pH8.5 |
| 5 | 1 | reservoir | PEG8000 | 13-15 (%) | |
| 6 | 1 | reservoir | magnesium acetate | 0.2 (M) |
