1PV7

Crystal structure of lactose permease with TDG

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-17
Detector	MARRESEARCH
Wavelength(s)	0.9150
Spacegroup name	P 21 21 21
Unit cell lengths	101.351, 125.845, 188.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 * - 3.600
R-factor	0.271
Rwork	0.271
R-free	0.29600
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	19.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.700
High resolution limit [Å]	3.600	3.600
Rmerge	0.128 *	0.443 *
Total number of observations	67117 *
Number of reflections	26017 *
Completeness [%]	90.5	85.6 *
Redundancy	2.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)	pH7.0
2	1	reservoir	PEG400	27-30 (%)
3	1	reservoir		200 (mM)
4	1	reservoir	1,6-hexanediol	3 (%)
5	1	reservoir	CHAPS	0.8 (mM)