1PV5
Structure of Protein of Unknown Function YwqG from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-06 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97926, 0.97945, 0.94644 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.801, 88.293, 40.103 |
| Unit cell angles | 90.00, 103.08, 90.00 |
Refinement procedure
| Resolution | 37.800 - 1.750 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.23500 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.800 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.094 | 0.262 |
| Number of reflections | 24853 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 93.7 | 63.8 |
| Redundancy | 9.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | magnesium chloride, HEPES, PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
