1PV1

Crystal Structure Analysis of Yeast Hypothetical Protein: YJG8_YEAST

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-03
Detector	BRANDEIS - B1
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	121.366, 66.929, 171.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.300
Rwork	0.255
R-free	0.29820
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.609
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.079	0.383
<I/σ(I)>	11.8
Completeness [%]	86.7	69.4
Redundancy		8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	Ammonium Acetate, Sodium acetate, PEG 4000, Glycerol , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K