1PUU
Mistletoe lectin I in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9073 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 106.278, 106.278, 312.329 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.2413 |
| Rwork | 0.207 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.560 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.970 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.036 | 0.368 |
| Number of reflections | 47510 | |
| <I/σ(I)> | 36.3 | 3.93 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 2.5 | 293 | ammonium sulphate, glycin/HCl, lactose, pH 2.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
