1PU4
Crystal structure of human vascular adhesion protein-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8110 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 225.916, 225.916, 218.668 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.21883 |
| Rwork | 0.217 |
| R-free | 0.25364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ksi |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.771 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.020 | 0.464 |
| Number of reflections | 52367 | |
| <I/σ(I)> | 13.2 | 6 |
| Completeness [%] | 95.9 | 97.2 |
| Redundancy | 14.1 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | potassium/sodium tartrate, imidazole, sodium chloride, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
