1PTV

CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH PHOSPHOTYROSINE

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1994-02
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	88.200, 88.200, 103.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.172
Rwork	0.172
RMSD bond length	0.017
RMSD bond angle	1.920
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Rmerge	0.035
Total number of observations	146758 *
Number of reflections	21436
Completeness [%]	99.7
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		4 *	Barford, D., (1994) J. Mol. Biol., 239, 726. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PTP1B	10 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir	magnesium acetate	0.2 (M)
4	1	reservoir	PEG8000	12-16 (%(w/v))