1PTR

PROTEIN KINASE C DELTA CYS2 DOMAIN COMPLEXED WITH PHORBOL-13-ACETATE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-01-05
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	32.400, 63.500, 65.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.194
Rwork	0.194
RMSD bond length	0.013 *
RMSD bond angle	3.200
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200
Rmerge	0.060
Number of reflections	3215
Completeness [%]	94.6
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	4 *	used as seeds *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	ammonium sulfate	55 (%)
3	1	drop	phosphate	0.1 (M)
4	1	drop	DTT	1 (mM)
5	1	reservoir	isopropanol	25 (%)
6	1	reservoir	ammonium acetate	0.2 (M)
7	1	reservoir	sodium phosphate	0.1 (M)