1PTQ

PROTEIN KINASE C DELTA CYS2 DOMAIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Detector technology	IMAGE PLATE
Collection date	1994-10-29
Detector	FUJI
Wavelength(s)	1.2826,1.2818,1.2613
Spacegroup name	C 2 2 21
Unit cell lengths	32.400, 63.500, 65.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.950
R-factor	0.196
Rwork	0.196
RMSD bond length	0.014 *
RMSD bond angle	2.900
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.950
Rmerge	0.060
Number of reflections	4073
Completeness [%]	89.0
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	4 *	used as seeds *
1	Vapor diffusion, hanging drop *	6.8 *	4 *	used as seeds *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	ammonium sulfate	55 (%)
3	1	drop	phosphate	0.1 (M)
4	1	drop	DTT	1 (mM)
5	1	reservoir	isopropanol	25 (%)
6	1	reservoir	ammonium acetate	0.2 (M)
7	1	reservoir	sodium phosphate	0.1 (M)