1PST

CRYSTALLOGRAPHIC ANALYSES OF SITE-DIRECTED MUTANTS OF THE PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER SPHAEROIDES

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	138.000, 77.500, 141.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 3.000
R-factor	0.218
Rwork	0.218
RMSD bond length	0.013
RMSD bond angle	3.350
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	3.000 *
Rmerge	0.103 *
Total number of observations	67299 *
Number of reflections	22184 *
Completeness [%]	71.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		taken from Allen, J.P. et al (1986). Proc. Natl. Acad. Sci., 83, 8589-8593. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
10	1	reservoir	PEG4000	22 (%)
11	1	reservoir	Tris chloride	15 (mM)
12	1	reservoir	EDTA	1 (mM)
13	1	reservoir		0.1 (%)
2	1	drop		0.36 (M)
3	1	drop	heptane triol	3.9 (%(w/v))
4	1	drop	PEG4000	12 (%(w/v))
5	1	drop	lauryldmethylamine oxide	0.06 (%)
6	1	drop	Tris chloride	15 (mM)
7	1	drop	EDTA	1 (mM)
8	1	drop		0.1 (%)
9	1	reservoir		0.6 (M)