1PSS
CRYSTALLOGRAPHIC ANALYSES OF SITE-DIRECTED MUTANTS OF THE PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER SPHAEROIDES
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 138.000, 77.500, 141.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 3.000 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 3.410 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.000 * |
| Rmerge | 0.077 * |
| Total number of observations | 61853 * |
| Number of reflections | 21518 * |
| Completeness [%] | 68.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | taken from Allen, J.P. et al (1986). Proc. Natl. Acad. Sci., 83, 8589-8593. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | PEG4000 | 22 (%) | |
| 11 | 1 | reservoir | Tris chloride | 15 (mM) | |
| 12 | 1 | reservoir | EDTA | 1 (mM) | |
| 13 | 1 | reservoir | 0.1 (%) | ||
| 2 | 1 | drop | 0.36 (M) | ||
| 3 | 1 | drop | heptane triol | 3.9 (%(w/v)) | |
| 4 | 1 | drop | PEG4000 | 12 (%(w/v)) | |
| 5 | 1 | drop | lauryldmethylamine oxide | 0.06 (%) | |
| 6 | 1 | drop | Tris chloride | 15 (mM) | |
| 7 | 1 | drop | EDTA | 1 (mM) | |
| 8 | 1 | drop | 0.1 (%) | ||
| 9 | 1 | reservoir | 0.6 (M) |
