1PSN

THE CRYSTAL STRUCTURE OF HUMAN PEPSIN AND ITS COMPLEX WITH PEPSTATIN

Experimental procedure

Source type	ROTATING ANODE
Detector technology	AREA DETECTOR
Collection date	1994-05-12
Detector	SAN DIEGO MULTIWIRE DETECTION SYSTEM
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	71.970, 151.590, 41.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
Rwork	0.188
RMSD bond length	0.010
RMSD bond angle	2.810 *
Data reduction software	SAN (DIEGO SOFTWARE)
Refinement software	GROMOS

Data quality characteristics

	Overall
High resolution limit [Å]	1.780 *
Rmerge	0.075 *
Total number of observations	99655 *
Number of reflections	33057
Completeness [%]	75.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium sulfate	40 (%sat)
3	1	reservoir	Na acetate	100 (mM)
4	1	reservoir	MPD	5 (%)