1PSJ

ACIDIC PHOSPHOLIPASE A2 FROM AGKISTRODON HALYS PALLAS

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1992-03
Detector	SIEMENS
Spacegroup name	P 61
Unit cell lengths	83.570, 83.570, 32.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.157
Rwork	0.157
R-free	0.22800
Structure solution method	MR
RMSD bond length	0.013
RMSD bond angle	2.690 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.810
High resolution limit [Å]	2.000 *
Rmerge	0.042 *
Number of reflections	7861 *
Completeness [%]	87.0 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.94 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		0.05 (M)
3	1	drop	2,5-hexanediol	10 (%(v/v))
4	1	reservoir	2,5-hexanediol	65 (%)