1PSC

PHOSPHOTRIESTERASE FROM PSEUDOMONAS DIMINUTA

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	129.500, 91.400, 69.400
Unit cell angles	90.00, 91.90, 90.00

Refinement procedure

Resolution	30.000 - 2.000
Rwork	0.152
RMSD bond length	0.016
RMSD bond angle	16.100 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000	2.000 *
Rmerge	0.040 *	0.100 *
Total number of observations	151079 *
Number of reflections	49624	5550 *
Completeness [%]	91.0 *	82 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	reservoir	PEG8000	10 (%)
4	1	reservoir		5 (mM)
5	1	reservoir	diethyl 4-methylbenzylphosphonate	1 (%)
6	1	reservoir	CHES	50 (mM)