1PS5
STRUCTURE OF THE MONOCLINIC C2 FORM OF HEN EGG-WHITE LYSOZYME AT 2.0 ANGSTROMS RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 42.944, 53.143, 50.211 |
| Unit cell angles | 90.00, 97.32, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iee |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.500 * |
| Data reduction software | DENZO ((DENZO)) |
| Data scaling software | SCALEPACK ((DENZO)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 7305 | |
| <I/σ(I)> | 15.7 | 7.4 |
| Completeness [%] | 95.5 | 79.7 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 * | 313 | 2 M Lithium Sulfate, 8% MPD , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 313K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | 350 (mM) | ||
| 3 | 1 | drop | Tris | 2.5 (mM) | pH7.1 |
| 4 | 1 | drop | 0.02 (%) |
