1PRW
Crystal structure of bovine brain Ca++ calmodulin in a compact form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.320, 35.750, 68.030 |
| Unit cell angles | 90.00, 117.19, 90.00 |
Refinement procedure
| Resolution | 14.590 - 1.700 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.23500 * |
| Structure solution method | SIRSAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.010 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.062 * | 0.322 * |
| Number of reflections | 12598 * | |
| <I/σ(I)> | 14.8 | 4.28 |
| Completeness [%] | 92.4 * | 90.7 |
| Redundancy | 3.6 | 2.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 23 * | PEG 6000, sodium acetate, glycerol, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10 (mM) | ||
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | PEG6000 | 40 (%) | |
| 4 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 5 | 1 | reservoir | 10 (mM) | pH5.6 |
