1PRP
CRYSTAL STRUCTURE OF D(CGCGAATTCGCG) COMPLEXED WITH PROPAMIDINE, A SHORT-CHAIN HOMOLOGUE OF THE DRUG PENTAMIDIN
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 289 |
| Detector technology | AREA DETECTOR |
| Detector | XENTRONICS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.000, 40.880, 67.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.083 |
| Total number of observations | 11478 * |
| Number of reflections | 4021 |
| Completeness [%] | 90.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 286 * | pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MPD | 14.6 (%) | |
| 2 | 1 | drop | propamidine | 1.5 (mM) | |
| 3 | 1 | drop | 30 (mM) | ||
| 4 | 1 | drop | DNA | 0.9 (mM) | |
| 5 | 1 | reservoir | MPD | 40 (%) | |
| 6 | 1 | reservoir | sodium cacodylate | 30 (mM) |
