1PRN

REFINED STRUCTURE OF PORIN FROM RHODOPSEUDOMONAS BLASTICA AND COMPARISON WITH THE PORIN FROM RHODOBACTER CAPSULATUS

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	104.300, 104.300, 124.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.960
R-factor	0.176
Rwork	0.176
RMSD bond length	0.011
RMSD bond angle	1.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	1.960 *	1.960 *
Rmerge	0.078 *
Total number of observations	291952 *
Number of reflections	35847 *
Completeness [%]	75.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		300 (mM)
4	1	drop	n-octyltetraoxyethylene	0.6 (%(w/v))
5	1	drop		3 (mM)
6	1	drop	PEG600	10-18 (%)
7	1	reservoir	PEG600	30-38 (%(w/v))