1PRH

THE X-RAY CRYSTAL STRUCTURE OF THE MEMBRANE PROTEIN PROSTAGLANDIN H2 SYNTHASE-1

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	99.400, 210.300, 233.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.500
R-factor	0.267
Rwork	0.267
R-free	0.31600
RMSD bond length	0.014
RMSD bond angle	3.200
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	3.400 *
Rmerge	0.076 *
Total number of observations	112745 *
Number of reflections	32349 *
Completeness [%]	96.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium phosphate	20 (mM)
10	1	reservoir	PEG4000	4-8 (%)
2	1	drop		100-200 (mM)
3	1	drop	beta-octyl glucopyranoside	0.6 (%(w/v))
4	1	drop	flurbiprofen	0.1 (mM)
5	1	drop	PEG4000	2-4 (%)
6	1	reservoir	sodium phosphate	20 (mM)	2-4 times concentrate
7	1	reservoir		100-200 (mM)	2-4 times concentrate
8	1	reservoir	beta-octyl glucopyranoside	0.6 (%(w/v))	2-4 times concentrate
9	1	reservoir	flurbiprofen	0.1 (mM)	2-4 times concentrate