1PQY

Crystal structure of formyl-coA transferase yfdW from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-23
Detector	MARRESEARCH
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	92.261, 69.539, 73.109
Unit cell angles	90.00, 108.64, 90.00

Refinement procedure

Resolution	27.990 - 2.350
Rwork	0.177
R-free	0.21100
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.990	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.093	0.180
<I/σ(I)>	24.3	20
Completeness [%]	98.3	97.8
Redundancy	7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	MPD, calcium chloride, Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	MPD, calcium chloride, Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	MPD, calcium chloride, Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K