1PQV
RNA polymerase II-TFIIS complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Detector technology | CCD |
| Collection date | 2003-03-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 218.900, 395.300, 281.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.089 * | 0.355 * |
| Number of reflections | 115508 | 9618 * |
| Completeness [%] | 96.2 | 81 |
| Redundancy | 2.9 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 20 * | sodium-ammonium tartrate, Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | reservoir | ammonium-sodium tartrate | 850 (mM) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) |
