1PQM

T4 Lysozyme Core Repacking Mutant V149I/T152V/TA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-08-22
Detector	RIGAKU RAXIS IV
Spacegroup name	P 32 2 1
Unit cell lengths	59.748, 59.748, 95.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.500 - 1.520
R-factor	0.179
Rwork	0.177
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.016 *
RMSD bond angle	2.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.881	1.550
High resolution limit [Å]	1.520 *	1.450
Rmerge	0.058	0.096
Number of reflections	31288
<I/σ(I)>	6.9	6.9
Completeness [%]	93.5 *	71 *
Redundancy	4.4	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol