1PQJ

T4 LYSOZYME CORE REPACKING MUTANT A111V/CORE10/TA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-07-27
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	59.520, 59.520, 95.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.700 - 1.900
Rwork	0.184
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	lL63
RMSD bond length	0.016 *
RMSD bond angle	2.600 *

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.900 *
Rmerge	0.061 *
Completeness [%]	99.0 *	100 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol