1PQI

T4 LYSOZYME CORE REPACKING MUTANT I118L/CORE7/TA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2001-10-11
Detector	RIGAKU RAXIS IV
Spacegroup name	P 32 2 1
Unit cell lengths	60.001, 60.001, 95.615
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.830 - 1.560 *
R-factor	0.204
Rwork	0.200
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.016 *
RMSD bond angle	2.700 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.820	1.640
High resolution limit [Å]	1.570	1.570
Rmerge	0.049	0.204
Number of reflections	29013
<I/σ(I)>	7	3.5
Completeness [%]	91.1 *	91 *
Redundancy	4.5	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol