1PQD

T4 LYSOZYME CORE REPACKING MUTANT CORE10/TA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-08-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	59.972, 59.972, 96.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.300 - 1.650
R-factor	0.183
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.016 *
RMSD bond angle	2.700 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.300
High resolution limit [Å]	1.650
Rmerge	0.076 *
Number of reflections	23982
Completeness [%]	96.9 *	85 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7		Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol