1PQ7

Trypsin at 0.8 A, pH5 / borax

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-20
Detector	MARRESEARCH
Wavelength(s)	0.8110
Spacegroup name	P 1
Unit cell lengths	32.869, 37.022, 39.780
Unit cell angles	102.89, 104.59, 102.37

Refinement procedure

Resolution	30.000 - 0.800
Rwork	0.109
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000 *	0.810
High resolution limit [Å]	0.800	0.800
Rmerge	0.033 *
Number of reflections	165882 *
<I/σ(I)>	15	6.2
Completeness [%]	92.7	73
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5	293	Rypniewski, W.R., (1993) Protein Eng., 6, 341. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12.5 (mg/ml)
2	1	drop	ammonium sulfate	0.7 (M)
3	1	drop	citrate	50 (mM)
4	1	reservoir	ammonium sulfate	1.4 (M)
5	1	reservoir	sodium citrate	0.1 (M)