1PPJ

Bovine cytochrome bc1 complex with stigmatellin and antimycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-06-26
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	128.530, 168.748, 231.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.530 - 2.100
R-factor	0.224
Rwork	0.224
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1BE3 2BCC
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	250.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.149	0.879
Number of reflections	285923
<I/σ(I)>	18.689	2.819
Completeness [%]	98.1	94.3
Redundancy	5.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.7	277	PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE , pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 6.70
1		6.7	277	PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE , pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 6.70
1		6.7	277	PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE , pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 6.70
1		6.7	277	PEG-3350, JEFFAMINE, GLYCEROL, CACODYLATE, HEXYLGLUCOSIDE , pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 6.70