1PP6
VVA2 (STRIP CRYSTAL FORM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 133 |
| Detector technology | CCD |
| Collection date | 2002-06-10 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.672, 80.679, 107.853 |
| Unit cell angles | 90.00, 105.33, 90.00 |
Refinement procedure
| Resolution | 28.540 - 3.200 |
| Rwork | 0.264 |
| R-free | 0.34800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pp0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.540 | 3.240 |
| High resolution limit [Å] | 3.180 | 3.200 |
| Rmerge | 0.124 | 0.472 |
| Number of reflections | 19692 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 98.3 | 99.9 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium cacodylate, PEG 8000, glycerol, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
