1POW
THE REFINED STRUCTURES OF A STABILIZED MUTANT AND OF WILD-TYPE PYRUVATE OXIDASE FROM LACTOBACILLUS PLANTARUM
Experimental procedure
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 121.600, 155.400, 167.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.600 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.2 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 20 (mg/ml) | |
| 2 | 1 | drop | potasssium phosphate | 50 (mM) | |
| 3 | 1 | drop | glycerol | 5 (%(v/v)) | |
| 4 | 1 | drop | ammonium sulphate | 0.5 (M) | |
| 5 | 1 | drop | ThDP | 1 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.95 (M) | |
| 7 | 1 | reservoir | potassium phosphate | 50 (mM) | |
| 8 | 1 | reservoir | glycerol | 10 (%(v/v)) |
