1POT

SPERMIDINE/PUTRESCINE-BINDING PROTEIN COMPLEXED WITH SPERMIDINE (MONOMER FORM)

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-02-01
Detector	MACSCIENCE
Spacegroup name	I 41
Unit cell lengths	130.300, 130.300, 38.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.198 *
Rwork	0.198
RMSD bond length	0.013
RMSD bond angle	0.037
Data reduction software	WELMS
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.800
Rmerge	0.091
Total number of observations	246531 *
Number of reflections	24579
Completeness [%]	87.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	15 (%)
2	1	drop	HEPES	10 (mM)