1POD
STRUCTURES OF FREE AND INHIBITED HUMAN SECRETORY PHOSPHOLIPASE A2 FROM INFLAMMATORY EXUDATE
Experimental procedure
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 76.300, 76.300, 90.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.100 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.300 |
| Phasing software | X-PLOR |
| Refinement software | PROFFT |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 4 | 1 | drop | 2.5 (M) | ||
| 5 | 1 | drop | beta-octyl-glucopyranoside | 0.5 (mM) | |
| 6 | 1 | reservoir | 4.9 (M) | ||
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) |
