1POC
CRYSTAL STRUCTURE OF BEE-VENOM PHOSPHOLIPASE A2 IN A COMPLEX WITH A TRANSITION-STATE ANALOGUE
Experimental procedure
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 89.500, 89.500, 132.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.100 |
| Phasing software | X-PLOR |
| Refinement software | PROFFT |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 3 | 1 | drop | diC8(2Ph)PE | 3 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | 30 (%sat) | ||
| 6 | 1 | reservoir | 60 (%sat) | ||
| 7 | 1 | reservoir | Tris-HCl | 0.2 (M) |
