1PNG

CRYSTAL STRUCTURE OF PEPTIDE-N(4)-(N-ACETYL-BETA-D-GLUCOSAMINYL) ASPARAGINE AMIDASE AT 2.2 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	87.160, 125.100, 79.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.100 - 2.200
Rwork	0.183
RMSD bond length	0.013
RMSD bond angle	1.970

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Total number of observations	95693 *
Number of reflections	22074 *
Completeness [%]	98.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.3 *	10 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.7 (mg/ml)
2	1	drop	cacodylate	5.6 (mM)
3	1	drop	dithiothreitol	1.1 (mM)
4	1	drop	ammonium sulfate	0.13 (M)
5	1	drop	PEG3350	13.3 (%)
6	1	drop	sodium acetate	0.04 (M)
7	1	reservoir	ammonium sulfate	0.4 (M)
8	1	reservoir	PEG3350	32 (%)
9	1	reservoir	sodium acetate	0.1 (M)