1PNF
PNGASE F COMPLEX WITH DI-N-ACETYLCHITOBIOSE
Experimental procedure
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.110, 123.080, 77.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.670 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 |
| High resolution limit [Å] | 1.960 |
| Rmerge | 0.044 * |
| Number of reflections | 28801 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | N,N'-diacetylchitobiose | 30:1 molar ratio to PNGase F | |
| 2 | 1 | 1 | PNGase F | ||
| 3 | 1 | 1 | lyophilized disaccharide | ||
| 4 | 1 | 1 | protein | 10 (mg/ml) | |
| 5 | 1 | 1 | glycerol | 50 (%) |
