1PND
ACCURACY AND PRECISION IN PROTEIN CRYSTAL STRUCTURE ANALYSIS: TWO INDEPENDENT REFINEMENTS OF THE STRUCTURE OF POPLAR PLASTOCYANIN AT 173K
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Detector technology | AREA DETECTOR |
| Detector | ENRAF-NONIUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.180, 46.290, 56.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.600 |
| R-factor | 0.135 * |
| Rwork | 0.153 |
| RMSD bond length | 0.153 |
| RMSD bond angle | 0.011 |
| Refinement software | EREF |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 * | taken from Chapman, G.V. et al (1977). J. Mol. Biol., 110, 187-189. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | reservoir | sodium phosphate | 0.1 (M) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.6 (M) |
