1PMP

CRYSTALLOGRAPHIC STUDIES ON A FAMILY OF CELLULAR LIPOPHILIC TRANSPORT PROTEINS. REFINEMENT OF P2 MYELIN PROTEIN AND THE STRUCTURE DETERMINATION AND REFINEMENT OF CELLULAR RETINOL-BINDING PROTEIN IN COMPLEX WITH ALL-TRANS-RETINOL

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	91.800, 99.500, 56.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 2.700
R-factor	0.268
Rwork	0.268
RMSD bond length	0.009
RMSD bond angle	0.022 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.085 *
Total number of observations	85809 *
Number of reflections	11665 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		Bergfors, T., (1987) J. Mol. Biol., 198, 357. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MES/Tris buffer	20 (mM)
2	1	drop	P2	15 (mg/ml)
3	1	drop	PEG4000	20 (%)
4	1	reservoir	PEG	20 (%)
5	1	reservoir	MES/Tris	20 (mM)